Types of Bioremediation

types of bioremediation

Bioremediation is an environmental management technique that uses biological organisms, primarily microorganisms and plants, to reduce, eliminate, contain, or transform pollutants. This method is commonly used to address pollution in soil, water, and air, and is often a more sustainable and cost-effective solution than many traditional techniques.

The idea behind bioremediation is to boost the natural processes of biodegradation, in which living organisms break down harmful substances. This is usually achieved either by enhancing the growth of existing, naturally occurring bacteria and fungi (biostimulation) or by adding specific types of bacteria and fungi that are known to degrade certain pollutants (bioaugmentation).

History of Bioremediation

History of Bioremediation

The concept of using living organisms for environmental cleanup, the foundation of bioremediation, has been a part of human history for centuries. However, the formal study and application of bioremediation techniques, as we know them today, only started gaining attention in the mid to late 20th century.

The biological treatment of waste, particularly in the form of wastewater treatment, has been practiced since the late 19th century. Early sewage systems used biological methods like anaerobic digestion, but these were not recognized as bioremediation at the time.

In the 1960s and 70s, as the environmental movement grew, there was an increasing awareness of the need for more sustainable methods to deal with pollution. The modern concept of bioremediation began to take shape during this period, although the term itself wasn’t widely used until the 1980s.

The application of bioremediation got significant attention after the Exxon Valdez oil spill in 1989 in Alaska. Researchers used fertilizers to stimulate the growth of indigenous bacteria, which helped to accelerate the natural process of biodegradation and proved successful in degrading the oil. This was a landmark event in the history of bioremediation, showing its potential on a large scale.

Exxon Valdez oil spill in 1989

Exxon Valdez Oil Spill in 1989

In the 1990s and 2000s, bioremediation technologies developed rapidly with advancements in microbiology, genetics, and environmental engineering. Scientists were able to identify, cultivate, and manipulate bacteria that were capable of degrading specific pollutants. This period also saw the rise of phytoremediation, the use of plants to remediate contaminated soils and water.

In the 21st century, bioremediation continues to evolve with advancements in genetic engineering and our understanding of microbial communities. For example, the emerging field of metagenomics, which involves studying the genetic material of entire communities of microbes, provides a powerful tool for discovering new bioremediation strategies.

Bioremediation techniques have been successfully applied in various scenarios, from oil spill cleanups to the treatment of industrial waste, contaminated soil, and groundwater. They’ve proven to be an effective and environmentally friendly approach to managing and mitigating pollution, and research in this field is ongoing, aiming to make bioremediation more efficient and applicable to a broader range of pollutants. You can take a look at other daily life examples of bioremediation in Bioremediation Examples in Real-life.

Bioremediation Factors

Several factors influence the effectiveness of bioremediation. These factors can affect the survival and metabolic activity of the organisms involved in the process, and hence, the rate and extent of pollutant degradation. Here are some of the key factors:

1. Type and Concentration of Pollutants: Different pollutants require different organisms or methods for effective bioremediation. Some pollutants may be more resistant to biodegradation than others, and high concentrations of pollutants can inhibit bioremediation processes.

2. Presence of Microorganisms: The presence of suitable microorganisms that can metabolize the pollutants is crucial. Different microorganisms have different capabilities, and some may be more efficient at degrading certain pollutants than others.

3. Environmental Conditions: The physical and chemical conditions of the environment can significantly influence bioremediation. This includes factors like temperature, pH, moisture content, and the presence of oxygen (aerobic conditions often enhance the biodegradation of many pollutants).

4. Nutrient Availability: The availability of nutrients such as nitrogen, phosphorus, and potassium can impact microbial growth and activity. If these nutrients are deficient, biostimulation can be used to enhance their levels.

5. Bioavailability of Pollutants This refers to the extent to which pollutants are accessible to the microorganisms. If pollutants are tightly bound to soil particles or trapped in certain structures, they may not be readily available for degradation.

6. Presence of Inhibitory Substances: Certain substances can inhibit microbial activity, either by being toxic to the microorganisms or by interfering with their metabolic processes.

7. Time: Bioremediation generally takes longer than traditional remediation methods. The exact time required depends on all the above factors.

8. Site Characteristics: The specific characteristics of the site, such as soil type, permeability, and groundwater flow, can influence the effectiveness of bioremediation.

Factors influencing bioremediation

Understanding these factors is essential to designing and implementing a successful bioremediation strategy. In many cases, a site-specific assessment is usually required to determine the most appropriate bioremediation approach.

Mechanism of Bioremediation

Bioremediation uses organisms, mainly microorganisms and plants, to degrade, transform, or immobilize pollutants in the environment. These organisms metabolize the pollutants for energy or accidentally during other metabolic activities, converting them into less harmful substances. Some organisms can accumulate pollutants in their tissues or convert them into forms that are not bioavailable, effectively reducing their environmental impact. The specific mechanism depends on the nature of the pollutant, site characteristics, and the organisms involved.

Mechanism of bioremediation

Types of Bioremediation

Bioremediation, the use of living organisms to treat environmental pollution, is categorized into various types based on the approach and organisms used. In-situ bioremediation treats contamination on-site, often by stimulating indigenous microorganisms. Ex-situ bioremediation involves removing polluted material to treat it elsewhere. Other types include bioaugmentation, which introduces pollutant-degrading bacteria to the site; biostimulation, which enhances the activity of native bacteria; phytoremediation, which employs plants for cleanup; and mycoremediation, which utilizes fungi for biodegradation.

Types of Bioremediation Based on Strategies

In-Situ Bioremediation

In-situ bioremediation refers to the process of cleaning up environmental pollution at the site where it occurred, without needing to remove the polluted materials to treat them elsewhere. This approach is often used for treating contaminated soils and groundwater. In-situ bioremediation generally involves the use of microorganisms to degrade, transform, or immobilize pollutants. It often leverages the naturally occurring microbial communities that are adapted to the specific conditions of the site.

  • Bioventing: Bioventing is a form of in-situ bioremediation that stimulates the natural in-place bacteria that are capable of degrading pollutants, especially petroleum hydrocarbons. The technique primarily involves supplying air or oxygen into the unsaturated zone of the soil (i.e., above the water table), which boosts microbial activity and enhances the natural biodegradation process. This method is most commonly used to remediate soils contaminated with petroleum hydrocarbons, such as gasoline, diesel, and heating oils. Bioventing is particularly effective for treating lighter, more volatile fractions of these fuels, such as the BTEX compounds (benzene, toluene, ethylbenzene, and xylene). The process usually involves the installation of a series of wells into the soil through which air or oxygen can be supplied. The air can be either injected into the soil or drawn through the soil by applying a vacuum to the wells. This helps to ensure the distribution of oxygen throughout the soil, stimulating the activity of aerobic bacteria.


  • Biosparging: Biosparging is an in-situ bioremediation technology that involves injecting air or oxygen into the saturated zone of the soil (i.e., below the water table). Similar to bioventing, biosparging aims to stimulate the activity of indigenous microorganisms capable of degrading pollutants, particularly petroleum hydrocarbons. Air or oxygen, and sometimes nutrients, are injected into the groundwater via a system of wells. The injected air travels up through the soil and groundwater, providing oxygen to the microorganisms. The increase in oxygen levels enhances the rate of biological degradation of the pollutants by these microorganisms.


  • Permeable Reactive Barrier (PRB):A Permeable Reactive Barrier (PRB) is an in-situ remediation technology that treats contaminated groundwater. Installed in the path of the groundwater plume, it contains reactive materials that degrade or capture pollutants. As groundwater flows through this permeable barrier, contaminants interact with the reactive materials, which may chemically or biologically degrade them, or adsorb them onto the barrier material. PRBs are typically used to treat contamination from heavy metals, radionuclides, and organic compounds. This method provides a long-term, passive remediation strategy, requiring minimal maintenance.

Permeable Reactive Barrier

Advantages of In-Situ Bioremediation

In-situ bioremediation, or treating contamination directly at the site, offers several advantages over other remediation methods:

  • Cost-Effective: In-situ bioremediation generally requires fewer resources, less equipment, and less labor than ex-situ methods, which involve excavating and transporting contaminated materials. This makes in-situ bioremediation a more cost-effective option in many cases.
  • Less Disruptive: Because the contamination is treated on-site, there is less disruption to the environment. There’s no need to dig up and transport contaminated soil, which can also result in secondary contamination issues.
  • Treats a Wide Range of Contaminants: In-situ bioremediation can be used to treat a wide range of organic contaminants, including petroleum hydrocarbons, solvents, and pesticides. Some in-situ bioremediation methods can also be used for certain types of inorganic contaminants.
  • No Residual Waste: Unlike some physical and chemical remediation methods, in-situ bioremediation doesn’t leave behind residual waste that needs to be managed and disposed of.
  • Public Acceptance: Because it is a natural process, bioremediation tends to be more publicly accepted compared to other remediation techniques.

However, in-situ bioremediation also has its limitations. The success of in-situ bioremediation depends on the nature of the contamination, the characteristics of the site, and the environmental conditions, and a detailed site assessment is typically required to determine if in-situ bioremediation is suitable. In addition, the process can take longer than other remediation methods and it can be challenging to control the treatment conditions and ensure the effective distribution of nutrients and other amendments in the environment.

Ex-Situ Bioremediation

Ex-situ bioremediation involves the removal of the contaminated materials from their original location and treating them elsewhere. The term “ex-situ” literally means “off-site.” This approach is typically used when the concentration of pollutants is too high, when the contamination is too deep, or when faster cleanup times are required.

There are several methods for ex-situ bioremediation:

  • Landfarming: Landfarming is an ex-situ bioremediation technique used primarily to treat soils contaminated with petroleum hydrocarbons and other organic pollutants. The process involves excavating the contaminated soil from its original site and spreading it in thin layers over a large, prepared surface area, called a landfarm. The landfarm site is usually lined with an impermeable material to prevent contaminants from leaching into the groundwater. Once spread, the soil is regularly tilled to mix and aerate it, which stimulates the growth and activity of indigenous microorganisms capable of degrading the contaminants. Over time, the contaminants are biodegraded, reducing their concentration to safe levels.


  • Biopiling: Biopiling is an ex-situ bioremediation technique used to treat soil contaminated with organic pollutants. The process involves excavating the contaminated soil, forming it into piles or “biopiles,” and stimulating microbial activity to degrade the pollutants. Biopiles are typically constructed over an impermeable liner and equipped with an aeration system to ensure proper oxygen supply for the microorganisms. Nutrients and moisture may also be added to boost microbial growth and pollutant degradation. Over time, the microorganisms break down the contaminants, thereby cleaning the soil.


  • Bioreactors: Bioreactors are engineered systems used for ex-situ bioremediation, designed to provide optimal conditions for microorganisms to degrade pollutants. They’re used to treat contaminated soil, water, or waste by manipulating factors such as temperature, pH, nutrient levels, and aeration to maximize microbial activity. The contaminated material is placed in the bioreactor, and conditions are closely monitored and adjusted to optimize the degradation of the target pollutants. Bioreactors can be very efficient at degrading pollutants and can treat a wide range of contaminants. They provide a high degree of control over the treatment process, allowing for faster cleanup times compared to in-situ methods.


  • Composting: Composting is an ex-situ bioremediation technique that employs the process of organic matter decomposition to treat contaminated soil. This method involves mixing the contaminated soil with organic matter, such as yard waste or manure, and a bulking agent to create a compost pile. The added organic matter provides a carbon source, while the bulking agent ensures optimal aeration. The compost pile is maintained at a certain temperature and moisture level to promote the growth of thermophilic microorganisms. These microorganisms degrade the organic pollutants while the organic matter decomposes.


Advantages of Ex-Situ Bioremediation

Ex-situ bioremediation techniques, which involve removing the contaminated materials from their original site and treating them elsewhere, offer several advantages:

  • Control Over Conditions: Ex-situ bioremediation provides more control over the treatment conditions. Factors such as temperature, moisture, nutrient levels, and oxygen supply can be closely regulated to maximize the rate of biodegradation.
  • Speed: Because the conditions can be optimized for biodegradation, ex-situ techniques can often clean up contamination faster than in-situ techniques.
  • Effectiveness: Ex-situ bioremediation can be more effective for certain types of contamination or in situations where the contaminant concentration is very high.
  • Versatility: Ex-situ bioremediation techniques can be used to treat a variety of contaminants and are not limited by the depth or accessibility of the contamination.
  • Monitoring: The progress of the bioremediation process can be more easily monitored and adjusted as needed.

However, ex-situ bioremediation also has its downsides. It can be more disruptive and costly than in-situ methods due to the need for excavation and transportation of contaminated materials. It can also result in secondary contamination issues if not managed properly. As with all remediation techniques, a thorough site assessment is needed to determine the most appropriate method.

Difference Between In-Situ and Ex-Situ Bioremediation

Difference Between In-Situ and Ex-Situ Bioremediation Techniques

Types of Bioremediation Based on Microorganisms

Bioremediation strategies can also be classified based on the types of microorganisms involved. The major categories include:

Bacterial Bioremediation

Bacterioremediation involves the use of bacteria to degrade or transform environmental pollutants. It is one of the most common types of bioremediation due to the diverse metabolic capabilities of bacteria. Some bacteria can degrade organic pollutants, such as petroleum hydrocarbons, pesticides, and solvents, while others can transform inorganic pollutants, such as heavy metals. Bacterioremediation can involve either biostimulation, which enhances the activity of indigenous bacteria, or bioaugmentation, which introduces pollutant-degrading bacteria into the environment.

Fungal Bioremediation (Mycoremediation)


Mycoremediation refers to the use of fungi, particularly white-rot fungi, to degrade environmental pollutants. Fungi produce enzymes, such as laccases and peroxidases, capable of breaking down complex organic compounds including polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), and even some heavy metals. In addition to degradation, fungi can immobilize contaminants, reducing their bioavailability and toxicity. Factors influencing its success include fungal species, contaminant type, and environmental conditions.



Phytoremediation refers to the use of plants to clean up environmental contamination. Some plants have the ability to accumulate, degrade, or stabilize pollutants in their tissues or the rhizosphere – the soil region near plant roots. This technique can be used to treat a variety of contaminants, including heavy metals, petroleum hydrocarbons, and organic compounds. In addition to improving soil and water quality, phytoremediation also helps in reducing soil erosion and promoting biodiversity. However, phytoremediation is a slow process and may not be suitable for sites with high levels of contamination or for pollutants that are toxic to plants.

Algal Bioremediation

algal bioremediation

Algal bioremediation, sometimes considered a subset of phytoremediation, utilizes algae to remove pollutants from the environment, particularly from water bodies. Algae are effective at taking up heavy metals and excess nutrients such as nitrogen and phosphorus from contaminated waters. This makes them particularly useful in treating industrial wastewater or in mitigating the effects of agricultural runoff that can lead to harmful algal blooms and eutrophication in bodies of water. Some algae also have the ability to degrade or transform organic pollutants, and certain algae can even metabolize and degrade oil, making them potentially useful in treating oil spills.

Protozoan Bioremediation

Protozoan bioremediation leverages protozoa, a group of single-celled organisms, to treat pollutants, especially in wastewater systems. Protozoa play a vital role in the removal of organic matter and pathogenic bacteria, contributing to the overall purification process. They serve as predators in microbial ecosystems, controlling bacterial populations and improving water quality. In addition, certain types of protozoa can degrade organic pollutants directly. Protozoan bioremediation is less commonly used than bacterial or fungal bioremediation, but it is an emerging field with potential applications in wastewater treatment and soil remediation.

Bioremediation based on types of organisms

Each type of microorganism-based bioremediation has its advantages and limitations, and the choice of which to use depends on the nature of the pollutant, the characteristics of the contaminated site, and the desired cleanup objectives.

Bioremediation Based on Types of Degradation

Bioremediation can also be categorized based on the types of degradation processes involved. Here are a few common types:

Aerobic Biodegradation

Aerobic Degradation and Anaerobic Degradation

This process involves the breakdown of pollutants by microorganisms in the presence of oxygen. Many organic pollutants, including petroleum hydrocarbons and many types of pesticides and solvents, can be degraded aerobically. The process often involves oxidation reactions and can lead to the complete mineralization of the pollutant, turning it into carbon dioxide and water

Anaerobic Biodegradation

This refers to the breakdown of pollutants by microorganisms in the absence of oxygen. Some pollutants, such as certain chlorinated solvents, can be degraded more effectively under anaerobic conditions. Anaerobic biodegradation processes often involve reduction reactions.

Cometabolic Degradation

cometabolic degradation

In this process, the degradation of the pollutant occurs as a side effect of the microorganism’s primary metabolic activities. The pollutant is not used as a source of carbon or energy, but it is degraded by enzymes that the microorganism produces to metabolize its primary substrates. Cometabolic degradation can be important for the treatment of pollutants that are not easily degraded, such as chlorinated solvents.

Reductive Dechlorination

This is a specific type of anaerobic biodegradation process in which chlorinated organic pollutants are degraded through a series of reduction reactions, removing chlorine atoms and replacing them with hydrogen atoms. This process is important for the treatment of pollutants such as polychlorinated biphenyls (PCBs) and certain types of pesticides.

Each type of degradation-based bioremediation has its advantages and limitations, and the choice of which to use depends on the nature of the pollutant, the environmental conditions, and the metabolic capabilities of the available microorganisms.

Other Types of Bioremediation


Biostimulation is a method of bioremediation that encourages the growth and activity of indigenous (naturally occurring) microorganisms to enhance the degradation of pollutants. This is often achieved by adding nutrients, electron acceptors, or other amendments to the environment to stimulate microbial metabolism.

Biostimulation can be applied in both in-situ and ex-situ bioremediation settings.

In in-situ biostimulation, nutrients, electron acceptors, or other amendments are added directly to the contaminated site in the environment, such as a contaminated soil or groundwater system. The purpose is to stimulate the activity of the indigenous microorganisms in their natural location to enhance the degradation of contaminants.

Ex-situ biostimulation involves removing the contaminated material from its original site and treating it in a controlled setting, such as in bioreactors or treatment beds. Here, the same principles apply: amendments are added to the contaminated material to stimulate the activity of the indigenous microorganisms and enhance contaminant degradation.

The choice between in-situ and ex-situ biostimulation depends on several factors, including the nature and extent of the contamination, the characteristics of the site, the behavior and properties of the contaminants, and practical and economic considerations.


Bioaugmentation is a method of bioremediation that involves the introduction of specific strains or consortia of microorganisms into a contaminated environment to enhance the degradation of pollutants. These added microorganisms are selected or engineered because of their ability to degrade the specific contaminants present in the environment.

Bioaugmentation can be used in both in-situ and ex-situ bioremediation processes.

In in-situ bioaugmentation, specific strains or consortia of microorganisms that can degrade the target pollutants are introduced directly into the contaminated site in the environment, such as a soil or groundwater system. The aim is to enhance the biological degradation of contaminants in their natural location.

In ex-situ bioaugmentation, the contaminated material is removed from its original site and treated in a controlled setting. The selected microorganisms are added to this material to enhance the degradation of contaminants. This could be in bioreactors, treatment beds, or other controlled environments.

The choice between in-situ and ex-situ bioaugmentation depends on various factors including the type and level of contamination, the characteristics of the site and the contaminants, the feasibility of introducing and maintaining the added microorganisms in the site, and practical and economic considerations.

Biostimulation and Bioaugmentation


Bioremediation is a powerful, eco-friendly approach for managing pollution, employing various strategies based on the nature of the contamination, the site, and the desired outcomes. In-situ and ex-situ techniques provide options for treating contamination on-site or in a controlled environment, respectively. Specific methods such as biostimulation, bioaugmentation, and use of permeable reactive barriers, offer different ways to enhance microbial activity and degradation of pollutants.

Despite its numerous advantages, the effectiveness of bioremediation is influenced by various environmental factors and requires careful planning, execution, and monitoring. With continued research and technology development, bioremediation is set to play an increasingly important role in addressing environmental pollution challenges.

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